Diagnostics
of Invasive Aspergillosis:
From
Experimental Models to Clinical Evaluation
Combined
IAAM and AsTeC Workshop
Bethesda
Hyatt Regency, Bethesda MD
November
17, 2008
8:30 AM to 5:00 PM
Click highlighted names to
download a PDF file of the speaker’s presentation
1.
Welcome and Greetings by NIAID (Dennis Dixon, Rory Duncan, Christine
Chiou)
2.
AsTeC and IAAM Project overview (Tom
Patterson, John
Wingard)
What is the clinical need?
·
How these projects can contribute to the early development
and clinical application of new diagnostic tests
·
How these two projects are highly interactive
·
What are the range and types of assays & targets that can be
evaluated
3.
Animal models and utility
Murine model (Scott
Filler and Laura Najvar)
Rat model
(Peter
Warn)
Colonization
model (Don
Sheppard)
4.
Markers of Infection – Preclinical experience
PCR
evaluation (Brian
Wickes and Monica Herrera)
Beta glucan/ GM (Nathan
Wiederhold)
Host
Response in Invasive Aspergillosis (Tom
Walsh)
5. Break
6. Selected early diagnostic
evaluations
Lateral
Flow Device (Chris
Thornton)
Multiplex,
Real-Time PCR for diagnostics (John
Thornback)
Viatrode diagnostics (Gary
Fujii)
PCR for
diagnostics (Gordon
Ramage)
Aspergillus Gene Expression in Exp.
Infection (Elaine
Bignell)
7.
Box Lunch
8.
What clinical samples we will have in the AsTeC
repository (John
Wingard)
Population
under surveillance and sampling strategies
Types
of samples collected
Types of
clinical information recorded to annotate the samples
9.
What testing we can do with the AsTeC samples
(Barbara
Alexander)
Participating
laboratories, capabilities, and personnel
Test
evaluations:
Reproducibility
testing
Comparative
testing
Interference
substance testing
10. Utilizing the AsTeC
resource
Website,
IRB approvals (Lindsey
Baden)
Submission
procedure (Ming-Hong
Nguyen)
Review
Process (Joseph
Wheat)
11. Work to date
Contamination
Testing (David
Denning)
DNA
extraction methods: Bead beating & lysis Buffer,
Blood vs tissue samples
(Angie
Caliendo and Ana Vallor)
Fungal
DNA stability during storage (Ana
Vallor and, David Denning)
12. Break
13. ISHAM Working Group: European Aspergillus PCR Initiative (J.
Peter Donnelly)
14. Regulatory Issues: FDA Perspective (Freddie
Poole)
15. Panel
Discussion plus Q&A (Moderators: Tom Patterson, John Wingard)
(Bala, Caliendo, Hillyard, Poole, Selepak, Thornback, Thornton, Verweij)
16. Next steps, Q & A (Tom Patterson, John Wingard)